cAMPNOMAD D1 Dopamine Receptor Cell Line

Reference: P70508

9.995,00

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD D1 Dopamine Receptor Cell Line contains HEK293/U2OS cells stably expressing the following constructs:

 

  • Human D1 Dopamine Receptor (DRD1) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

 

cAMPNOMAD DRD1 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to DRD1, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

The D1 dopamine receptor plays a pivotal role in brain function, actively influencing reward, motor control, and also cognition by stimulating adenylate cyclase. Predominantly located postsynaptically in areas like the striatum and cortex, D1 receptors actively contribute to motor control, reinforcing positive behaviors.

Furthermore, D1 receptor activation closely associates with positive reinforcement, affecting motivation and behaviors related to reward. This modulation extends to cognitive processes, actively impacting learning and memory. Dysregulation of D1 receptors implicates them in neuropsychiatric disorders, such as schizophrenia and addiction. Positioned mainly postsynaptically in key brain regions, including the striatum and also cortex, D1 receptors are integral to motor control. Their activation actively stimulates adenylate cyclase, initiating cellular responses and neurotransmitter release. In neuropsychiatric disorders like schizophrenia and addiction, researchers observe dysregulation of D1 receptors, underscoring their significance in these conditions. Healthcare professionals actively use medications targeting D1 receptors, including antipsychotic drugs, to manage these disorders effectively. As researchers actively explore the complex role of D1 receptors in neurological and psychiatric conditions, they gain valuable insights. This exploration actively aims to enhance treatment options, providing a deeper understanding of the receptor’s multifaceted involvement in brain function.

Understanding the intricacies of D1 receptors is paramount for actively developing effective therapeutic interventions and improving patient outcomes in neuropsychiatric disorders. The active investigation of D1 receptor function significantly contributes to advancing our comprehension of brain function and its relevance to mental health.

Click HERE to know more about DRD1 Receptor.
  • Reference: P70508
  • Nomad Biosensors: cAMP
  • GeneBank Accesion Number: NM000794
  • Size/Quantity: 2 vials containing 3x10⁶ cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice

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