D1 Dopamine Receptor Cell Line

cAMPNOMAD D1 Dopamine Receptor Cell Line

Reference: P70508

cAMPNOMAD D1 Dopamine Receptor Cell Line

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD D1 Dopamine Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human D1 Dopamine Receptor (DRD1) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD DRD1 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to DRD1, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

9.995,00€

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This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. Specifically, it is is highest in the dorsal striatum (caudate and putamen) and ventral striatum (nucleus accumbens and olfatory tubercle). But lower levels of DRD1 mRNA expression occur in the basolateral amygdala, cerebral cortex, septum, thalamus, and hypothalamus. This G-protein-coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. cAMPNOMAD D1 Dopamine Receptor Cell Line (DRD1 Cell Line) allows the measurement of the activity of ligands, such as Dopamine.

Click HERE to know more about DRD1 Receptor.

 

  • Reference: P70508
  • Nomad Biosensors: cAMP
  • GeneBank Accesion Number: NM000794
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice
Dopamine Receptors | GPCR | Nomad Cell Lines