Green Fluorescent B16-F10 Cell Line

Reference: P20121

Green Fluorescent B16-F10 Cell Line

Green Fluorescent B16-F10 Cell Line (aka LINTERNA B16-F10) has been developed through stable transfection of B16-F10 cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with Puromycin resistance gene:

LINTERNA B16-F10 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent B16-F10 Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.


Green Fluorescent B16-F10 Cell Line are suitable for both, in vitro and in vivo experimentation.

About B16-F10 Cell line:

B16F10 cell line is a high metastatic variant of the murine B16 mouse melanoma, from C57BL/6 (H-2b) mouse strain origin. This cell line is a mixture of both, adherent spindle and epithelial-like cells. B16F10 cell line is a well-established model for metastasis and it is applicable for the study of experimental cancer therapies.
Melanoma tumors express different tumor-associated antigens, which usually induce weak immune responses of short duration. Expression of both tumor-associated antigens p53 and TRP2 by melanoma cells raises the possibility of simultaneously targeting more than one antigen in a therapeutic vaccine.

About turboGFP protein:

tGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.

  • Reference: P20121
  • Size/Quantity: 3x106 cells / vial
  • Resistance Gene: Puromycin
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice