Green Fluorescent D3 Dopamine Receptor Cell Line

Reference: P30220

Green Fluorescent D3 Dopamine Receptor Cell Line

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent D3 Dopamine Receptor Cell Line contains SH-SY5Y cells stably expressing DRD3 Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent D3 Dopamine Receptor Cell Line allows to assay compounds, or analyze their capability to modulate DRD3 Receptor activation and the following redistribution process inside the cells. When a ligand binds to the DRD3 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent D3 Dopamine Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring DRD3 Receptor redistribution process in High Throughput Screening.


Size/Quantity: 2 vials containing 3×106 cells / vial


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DRD3 encodes the D3 subtype of the dopamine receptor. The D3 subtype inhibits adenylyl cyclase through inhibitory G-proteins. This receptor is present in phylogenetically older regions of the brain, suggesting that this receptor plays a role in cognitive and emotional functions. It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson’s disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). D3 agonists such as 7-OH-DPAT, pramipexole, and rotigotine, among others, display antidepressant effects in rodent models of depression. DRD3 antagonism has been shown to exacerbate locomotor activity in the brain. Green Fluorescent D3 Dopamine Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as Dopamine.

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  • Reference: P30220
  • DNA Accession Number: NM_000796
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice