Immortalized HUVEC

Reference: P10961-IM

2.085,00€

Immortalized HUVEC (IM-HUVEC) provided by Innoprot have been developed by immortalizing primary human umbilical vein endothelial cells with SV40 Large T antigen.

Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 5th passage while the SV40‐tranduced cells go beyond 30 passages. This immortalized cell line has been characterized by CD31 and VWF expression and tubule formation.

Each vial of Immortalized HUVEC contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HUVEC which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HUVEC culture.

Vascular endothelial cells contribute to the maintenance of vascular homeostasis. Vascular endothelial cells produce and secrete activators and inhibitors of the coagulation and fibrinolysis system. In addition, they mediate the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that regulate cell proliferation and control vessel wall tone. Human umbilical vein endothelial cells (HUVEC) are the type of cells most used in the study of endothelial cell processes. HUVEC have a “cobblestone” morphology, show positive staining for vWF/Factor VIII and CD-31, and the ability to take up acetylated low-density lipoprotein. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin and VCAM. Immortalized HUVEC are useful tools to stablish in vitro disease models for High Throughput and High Content Screening.