Mostrando 49–64 de 439 resultados

Fibroblast Medium

Fibroblast Medium

135,00460,00

Fibroblast Medium Kit is a complete medium designed for optimal growth of normal primary fibroblasts in vitro. Each Fibroblast Medium Kit consists of the next components:

  • 500 ml of Fibroblast basal medium
  • 10 ml of fetal bovine serum
  • 5 ml of Fibroblast Growth supplement
  • 5 ml of penicillin/streptomycin solution.
Fibroblast Medium-Plus

Fibroblast Medium Plus

140,00

Fibroblast Medium Plus Kit is a complete medium designed for optimal growth of normal primary cardiac and kidney fibroblasts in vitro. Each Fibroblast Medium Plus Kit consists of the next components:

  • 500 ml of Fibroblast basal medium-2
  • 25 ml of fetal bovine serum
  • 5 ml of Fibroblast Growth supplement-2
  • 5 ml of penicillin/streptomycin solution.
Fibroblast Medium

Fibroblast Medium-2

140,00

Fibroblast Medium Kit is a complete medium designed for optimal growth of normal primary fibroblasts in vitro. Each Fibroblast Medium Kit consists of the next components:

  • 500 ml of Fibroblast basal medium
  • 25 ml of fetal bovine serum
  • 5 ml of Fibroblast Growth supplement-2
  • 5 ml of penicillin/streptomycin solution.
FSHR Cell LlineFSHR Cell Lline

FluoHiTSeeker FSHR Cell Line

9.250,00

FluoHiTSeeker are cell lines stably co-expressing label-free GPCRs and the CRE-tGFP reporter gene. Each vial of FluoHiTSeeker FSHR Cell Line contains HEK293 cells stably expressing human Follicle Stimulating Hormone Receptor with no tag; and the CRE-tGFP reporter gene as a biosensor to monitor cAMP concentration changes. FluoHiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot FluoHiTSeeker FSHR Cell Line allows to assay compounds, or analyze their capability to modulate FSHR. When a ligand binds to the FSHR, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. Due to the presence of the CRE-tGFP reporter gene, this cell line allows to monitor cAMP concentration in living cells measuring fluorescence intensity. FluoHiTSeeker FSHR cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with rhFSH. The high reproducibility of this assay allows monitoring FSH receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

FluoHiTSeeker LHCGR Cell Line

FluoHiTSeeker LHCGR Cell Line

9.250,00

FluoHiTSeeker are cell lines stably co-expressing label-free GPCRs and the CRE-tGFP reporter gene. Each vial of FluoHiTSeeker LHCGR Cell Line contains HEK293 cells stably expressing human Luteinizing Hormone/Choriogonadotropin Receptor with no tag; and the CRE-tGFP reporter gene as a biosensor to monitor cAMP concentration changes. FluoHiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot FluoHiTSeeker LHCGR Cell Line allows to assay compounds, or analyze their capability to modulate LHCGR. When a ligand binds to the LHCGR, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. Due to the presence of the CRE-tGFP reporter gene, this cell line allows to monitor cAMP concentration in living cells measuring fluorescence intensity. FluoHiTSeeker LHCGR cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with rhCG. The high reproducibility of this assay allows monitoring FSH receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

Fluorescent Cytochrome c HEK293 Cell Line

Fluorescent Cytochrome c HEK293 Cell Line

1.995,00

A novel Fluorescent Cytochrome c HEK293 Cell Line (HEK293/Cytc-tGFP) has been developed through stable transfection for monitoring the cellular apoptosis level througth the Cytochrome c behavior and morphologicall changes in cell-based assays.
HEK293/Cytc-tGFP cell line was obtained by transfection of an expression vector for a fusion protein of mitochondria localization signal from Cytochrome and turboGFP.

Each vial of HEK293/Cytc-tGFP cell line contains 3 million cells. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

Fluorescent LMNB1 Cell Line

Fluorescent LMNB1 Cell Line

2.950,00

Novel Red Fluorescent LMNB1 Cell Lines have been developed through stable transfection with LMNB1 endogenously tagged with TagRFP in U2OS & SH-SY5Y cell lines. Both cell lines allow the monitorization of cellular apoptosis level through nuclear lamin B1 morphological changes in cell-based assays.

Each vial of both U2OS/LMNB1-TagRFP and SH-SY5Y/LMNB1-TagRFP cell lines contains 3 million cells. It is guaranteed that these cells will expand following the cell culture manual.

 

Size/Quantity: 1 vial containing 3×106 cells / vial

Fluorescent Mitochondria U2OS Cell Line

Fluorescent Mitochondria U2OS Cell Line

2.650,00

Fluorescent Mitochondria U2OS Cell Line has been developed transfecting an expression vector that encodes turbo green fluorescent protein (tGFP) fused to mitochondrial targeting sequence (MTS) derived from the subunit VIII of human cytochrome C oxidase. MTS is fused to the tGFP N-terminus and stably transfected into U2OS Cell Line. This cell line allows monitoring morphological changes in cells during mitochondrial damage.

Each vial of Fluorescent Mitochondria U2OS Cell Line contains 3 million cells. It is guaranteed that these cells will expand following the cell culture manual.

 

Size/Quantity: 1 vial containing 3×106 cells / vial

FUS/TLS Stress Granules Assay Cell LineFUS/TLS Stress Granules Assay Cell Line

FUS/TLS Stress Granules Assay Cell Line

9.995,00

Innoprot has developed a novel fluorescence cell-based assay cell line for High Content Screening that allows the quantification of FUS/TLS stress granules. This model consists in a U2OS cell line stably expressing fluorescent RNA-binding protein FUS/TLS (Fused in Sarcoma/Translocated in Sarcoma).

Innoprot offers this cell line as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing fluorescent FUS/TLS contains 3 million cells and Innoprot provides 2 vials of cells with each order. These cells proliferate in vitro and they are guaranteed to further expand for more than 30 passages following the instructions provided in the technical sheet.

Size/Quantity: 2 vials containing 3×106 cells / vial

Gamma Secretase Activity Assay Cell LineGamma Secretase Activity Assay Cell Line

Gamma Secretase Activity Assay Cell Line

9.995,00

Gamma Secretase Activity Assay Cell Line is an Alzheimer’s Disease in vitro model to identify γ-secretase activity inhibitors. It consists in U2OS cell line stably expressing green fluorescent APP-C99 construct, which contains the γ-secretase cleaving site. To perform the assay, cells are incubated with test compounds during 24 hours to evaluate their effects on APP Processing. After incubation, nucleus could be stained with DAPI and retained fluorescent APP vesicles are detected by fluorescence using image analysis algorithms.

Innoprot offers this assay as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing human tGFP-APP-C99 contains 3 million cells and Innoprot provides 2 vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

Gelatin Solution

Gelatin Solution

15,00

0.2% Gelatin Solution is a substrate for cell culture adhesion. Optimal conditions for attachment must be determined for each cell line and application. Recommended concentration for normal human cell attachment is 100-200 µg/cm2.

Product is stable for 2 years and remains unchanged from the date of receipt when stored at 2 – 8° C

Qty: 100 ml

Green Fluorescent A375 Cell LineGreen Fluorescent A375 Cell Line

Green Fluorescent A375 Cell Line

950,00

Green fluorescent A375 cell line (aka LINTERNA A375) has been developed through stable transfection of A375 cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with G418 resistance gene:

LINTERNA A375 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent A375 Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.

Green Fluorescent A549 Cell LineGreen Fluorescent A549 Cell Line

Green Fluorescent A549 Cell Line

950,00

Green Fluorescent A549 Cell Line (aka LINTERNA A549) has been developed through stable transfection of A549 cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with G418 resistance gene:

LINTERNA A549 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent A549 Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.

Green fluorescent alpha-synuclein SH-SY5Y Cell lineGreen Fluorescent alpha-synuclein SH-SY5Y Cell Line

Green fluorescent alpha-synuclein SH-SY5Y Cell line

2.950,00

A novel green fluorescent SH-SY5Y cell line has been developed through stable transfection with TagGPF2-α-synuclein. TagGFP2- α-synuclein cell line is stably-transfected and it is ready to use in cell-based assay applications. This stably transfected cell line provides consistent levels of expression, which helps to simplify the interpretation of the results. This cell line is intended to be used as an “in vitro” model for research studies.

Each vial of Green fluorescent alpha-synuclein SH-SY5Y Cell line contains 3 million cells. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

Green Fluorescent B1 Bradykinin Receptor Cell Line

Green Fluorescent B1 Bradykinin Receptor Cell Line

6.950,00

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent B1 Bradykinin Receptor Cell Line contains CHO-K1 cells stably expressing BDKRB1 tagged with tGFP.  These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent B1 Bradykinin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate BDKRB1 activation and the following redistribution process inside the cells. When a ligand binds to the BDKRB1, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent BDKRB1 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring B1 Bradykinin Receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

Green Fluorescent B16-F10 Cell LineLINTERNA B16-F10

Green Fluorescent B16-F10 Cell Line

950,00

Green Fluorescent B16-F10 Cell Line (aka LINTERNA B16-F10) has been developed through stable transfection of B16-F10 cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with Puromycin resistance gene:

LINTERNA B16-F10 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent B16-F10 Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.