Stable Cell Line Development

Innoprot offers stable Cell Line Development Service for a wide variety of genes including GPCRs, ion channels, proteases and kinases as well as various other enzymes, transmembrane proteins and adhesion molecules.

Expression of these genes could be a stable or inducible expression guaranteeing monoclonal cell lines using a single-cell printer from Cytena. Furthermore, at Innoprot we have developed a family of fluorescent reporter genes to measure Ca++, cAMP, DAG, RAS/RAF interaction, b-arrestin recruitment, Col1A1, etc.. which will allow you to perform directly your experiments on living cells.

Cell Line development service from Innoprot includes the next steps:

• Gene Synthesis
• Plasmid construction with the gene of interest
• Mammalian cell transfection with antibiotic selection
• Monoclonal Cells printing, screening and selection
• RT-PCR, IF study and Flow Cytometry Analysis
• Quantification of the number of receptors upon request
• Proof of cell stability during passages
• Selected clone expansion & Banking
• Mycoplasma, bacteria & Fungi screening

The time required to generate a new stable cell line is from 1 to 3 months. It depends on the cell line & the gene of interest.