{"version":"1.0","provider_name":"Innoprot","provider_url":"https:\/\/innoprot.com\/es\/","author_name":"Desarrollo","author_url":"https:\/\/innoprot.com\/es\/author\/desarrollo\/","title":"APOE4 Expressing Cell Lines &#8212; Innoprot","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"KA9zR0dzZ1\"><a href=\"https:\/\/innoprot.com\/es\/producto\/apoe4-expressing-cell-lines\/\">APOE4 Expressing Cell Lines<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/innoprot.com\/es\/producto\/apoe4-expressing-cell-lines\/embed\/#?secret=KA9zR0dzZ1\" width=\"600\" height=\"338\" title=\"\u00abAPOE4 Expressing Cell Lines\u00bb \u2014 Innoprot\" data-secret=\"KA9zR0dzZ1\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/innoprot.com\/wp-includes\/js\/wp-embed.min.js\n<\/script>\n","thumbnail_url":"http:\/\/innoprot.com\/wp-content\/uploads\/2025\/10\/astrocytes-apoe4.jpg","thumbnail_width":500,"thumbnail_height":500,"description":"Here we show a novel collection of stable cell lines overexpressing APOE4. These models provide a powerful tool for studying the molecular and cellular mechanisms underlying neurodegenerative diseases, including Alzheimer\u2019s disease.  HEK293, Immortalized Microglia, and Immortalized Astrocytes overexpressing ApoE4-FLAG are stably engineered to overexpress the ApoE4 isoform tagged with a C-terminal FLAG epitope, enabling easy detection and purification.\u00a0Protein overexpression has been validated by both immunofluorescence and Western blot. Additionally, active secretion of ApoE4 into the culture medium has been confirmedvia ELISA, ensuring functional expression and suitability for studies on lipid metabolism, neuroinflammation, and neurodegenerative disease mechanisms. Developed with precision and reproducibility in mind, our APOE4-expressing cell lines are optimized for a wide range of applications, from in vitro disease modeling to high-throughput screening."}