hAR Nuclear Translocation Assay Cell Line allows the analyzes of stimuli for their ability to modulate receptor nuclear translocation process. To perform this assay, you have to quantify the fluorescence distribution inside the cells with a High Content Imaging system.<\/p>\n
Human androgen receptor (hAR) is a ligand-dependent transcription factor responsible for the development of the male phenotype. The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. Androgenic hormones, such as testosterone or dihydrotestosterone binds the Androgen Receptor activating it.<\/p>\n
This reproducible assay allows monitoring androgen receptor nuclear translocation process in High Content Analysis and fluorescence microscope applications.<\/p>\n","protected":false},"excerpt":{"rendered":"
A novel HEK293 Cell line has been developed through stable transfectionof an expression vector for a fusion protein of turboGFP and human Androgen Receptor. The resultant cell line allows to monitor the dynamics of the hormone\/receptor complex in living cells and to assay for compounds inducing tGFP-hAR nuclear translocation and formation of nuclear foci.<\/p>\n
Innoprot hAR nuclear translocation Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate androgen receptor nuclear translocation process and quantifying the fluorescence distribution inside the cells. This highly reproducible assay allows monitoring androgen receptor nuclear translocation process in High Content Analysis and fluorescence microscope applications.<\/p>\n
Each vial of hAR Nuclear Translocation HEK293 Cell Line contains 3 million cells and Innoprot provides two vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.<\/p>\n","protected":false},"featured_media":1260,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[113],"product_tag":[320,322,319],"class_list":["post-1259","product","type-product","status-publish","has-post-thumbnail","product_cat-nuclear-receptor-cell-lines","product_tag-androgen-receptor","product_tag-har","product_tag-nuclear-translocation","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"yoast_head":"\n