{"id":1282,"date":"2019-01-16T18:53:45","date_gmt":"2019-01-16T17:53:45","guid":{"rendered":"http:\/\/innoprot.com\/?post_type=product&#038;p=1282"},"modified":"2019-12-12T11:35:18","modified_gmt":"2019-12-12T10:35:18","slug":"caspase-3-7-activity-assay-cell-line","status":"publish","type":"product","link":"https:\/\/innoprot.com\/es\/producto\/caspase-3-7-activity-assay-cell-line\/","title":{"rendered":"Caspase 3-7 Activity Assay Cell Line"},"content":{"rendered":"<p>The major executers in the apoptotic progam are proteases know as caspases (cysteine-dependent, aspartate- specific proteases). Caspase family orchestrate the changes of the cell during apoptotic process. Caspases exist as precursor, which, when activated iniciate the death program by destroying cellular key components and activating factors that mediate damage to the cells. A cleavage in the caspase 3 precursor induces it to activate and leads to the activated caspase-3 form. Our Caspase 3-7 Activity Assay Cell Line allows to monitor the caspase-3 activity level through the turboFP602 protein distribution in cell-based assays.<\/p>\n<p><a class=\"dt-pswp-item\" href=\"http:\/\/innoprot.com\/wp-content\/uploads\/2019\/01\/caspase-3-7-3.jpg\" data-dt-img-description=\"\" data-large_image_width=\"800\" data-large_image_height=\"439\" data-rel=\"lightbox-image-0\" data-rl_title=\"\" data-rl_caption=\"\" title=\"\"><img decoding=\"async\" class=\"size-medium wp-image-1285 alignright\" src=\"http:\/\/innoprot.com\/wp-content\/uploads\/2019\/01\/caspase-3-7-3-300x165.jpg\" alt=\"Caspase 3-7 Activity Assay Cell Line\" width=\"300\" height=\"165\" srcset=\"https:\/\/innoprot.com\/wp-content\/uploads\/2019\/01\/caspase-3-7-3-300x165.jpg 300w, https:\/\/innoprot.com\/wp-content\/uploads\/2019\/01\/caspase-3-7-3-768x421.jpg 768w, https:\/\/innoprot.com\/wp-content\/uploads\/2019\/01\/caspase-3-7-3-600x329.jpg 600w, https:\/\/innoprot.com\/wp-content\/uploads\/2019\/01\/caspase-3-7-3.jpg 800w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>Caspase 3-7 Activity Assay Cell Line contains a reporter polypeptide which comprises the next parts:<\/p>\n<ul>\n<li>A mitocondrial localization signal (MTS) which localizes the protein in the mitochondria<\/li>\n<li>The Red Fluorescent Protein FP602<\/li>\n<li>The caspase-3 cleavage sequence DEVD, which is the link between previous peptides<\/li>\n<\/ul>\n<div>In non-apoptotic cells, the tFP602 appears localized in mitochondria; in a perinuclear localization due to de MTS situated in the N-terminal of the construct. After promoting apoptosis, caspases are activated and cleave the specific caspase-clavage site (CCS) situated between the MTS and the tFP602 in the construct, resulting in liberation of tFP602 and entry into the nucleus. So, you can measure apoptosis by image analysis, quantifying the red fluorecence intensity inside the cell nucleus.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>A Novel Caspase 3-7 Activity Assay Cell Line has been developed in SH-SY5Y cell line through stable transfection with a caspase 3-7 activation reporter protein endogenously linked with FP602. When caspase 3-7 is activated, link is cleavaged and FP602 change its localization appearing in the nucleus. This cell line allows to monitor apoptosis by caspase 3-7 activation, quantifying the red fluorecence intensity inside the cell nucleus.<\/p>\n<p>Each vial of Caspase 3-7 Activity Assay Cell Line cell line contains 3 million cells. It is guaranteed that these cells will expand following the cell culture manual.<\/p>\n<p><strong>Size\/Quantity<\/strong>: 1 vial containing 3&#215;10<sup>6<\/sup> cells \/ vial<\/p>\n","protected":false},"featured_media":1284,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[116],"product_tag":[324,329,328],"class_list":["post-1282","product","type-product","status-publish","has-post-thumbnail","product_cat-stable-cell-lines-for-apoptosis-assays","product_tag-apoptosis","product_tag-caspase-3-7","product_tag-caspase-activation","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Caspase 3-7 Activity Assay Cell Line &#8212; Innoprot Apoptosis Cell Lines<\/title>\n<meta name=\"description\" content=\"Caspase 3-7 Activity Assay Cell Line allows to monitor the caspase-3 activation process in living cells by image analysis\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/innoprot.com\/es\/producto\/caspase-3-7-activity-assay-cell-line\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Caspase 3-7 Activity Assay Cell Line &#8212; 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