{"id":3005,"date":"2019-04-14T01:33:25","date_gmt":"2019-04-13T23:33:25","guid":{"rendered":"http:\/\/innoprot.com\/?post_type=product&#038;p=3005"},"modified":"2019-12-12T11:40:47","modified_gmt":"2019-12-12T10:40:47","slug":"green-fluorescent-immortalized-human-microglia","status":"publish","type":"product","link":"https:\/\/innoprot.com\/es\/producto\/green-fluorescent-immortalized-human-microglia\/","title":{"rendered":"Green Fluorescent Immortalized Human Microglia"},"content":{"rendered":"<p><em><strong>About HM:<\/strong><\/em><\/p>\n<p>Microglia is an important integral component of the neuro-glial cell network. They are present in the brain parenchyma from the early stage of development to the mature state. Microglia play an important role in brain immune surveillance. They can present antigens in the molecular context of MHC class II expression to CD-4 positive T cells. They are also capable of Fc-mediated phagocytosis, and share many common antigens with hemopoietic and tissue macrophages. Upon activation, they act as brain macrophages to clear tissue debris, damaged cells, or microbes when programmed cell death occurs during brain development or when the CNS is injured. Immortalized Human Microglia Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.<\/p>\n<p>&nbsp;<\/p>\n<p><em><strong>About turboGFP protein:<\/strong><\/em><\/p>\n<p>tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation\/ emission max = 482\/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><strong>Green Fluorescent Immortalized Human Microglia<\/strong> (IM-HM-tGFP) provided by Innoprot have been developed by immortalizing primary human microglia with SV40 Large T antigen and stably transfected with tGFP.<\/p>\n<p>Immortalized cells were controlled passaging side by side with the primary cells. These primary cells go into senescence after the 2nd passage while the SV40\u2010tranduced cells go beyond 30 passages.<\/p>\n<p>Each vial of <strong>Green Fluorescent Immortalized Human Microglia<\/strong> contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HM-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HM-tGFP culture.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Size\/Quantity:<\/strong> 1 million cells \/ vial<\/p>\n","protected":false},"featured_media":3006,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[118],"product_tag":[532,530,531],"class_list":["post-3005","product","type-product","status-publish","has-post-thumbnail","product_cat-fluorescent-immortalized-primary-cells","product_tag-green-fluorescent-cell-lines","product_tag-immortalized-cell-lines","product_tag-microglial-cells","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Green Fluorescent Immortalized Human Microglia &#8212; 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