{"id":7744,"date":"2022-12-29T23:28:55","date_gmt":"2022-12-29T22:28:55","guid":{"rendered":"http:\/\/innoprot.com\/?post_type=product&#038;p=7744"},"modified":"2022-12-29T23:28:55","modified_gmt":"2022-12-29T22:28:55","slug":"green-fluorescent-immortalized-haec","status":"publish","type":"product","link":"https:\/\/innoprot.com\/es\/producto\/green-fluorescent-immortalized-haec\/","title":{"rendered":"Green Fluorescent Immortalized HAEC"},"content":{"rendered":"<p><strong><em>About HAEC<\/em><\/strong><\/p>\n<p>Endothelial cells constitute the natural interface between the blood and the underlying tissue. Changes in endothelial cell function appear to play a key role in the pathogenesis of atherosclerosis. Endothelial cells synthesize and secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition to mediators that influence the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation and modulate vessel wall tone. Human aortic endothelial cells (HAEC) produce antithrombotic and thrombotic factors such as t-PA and PAI-1. They respond to TNFalpha by modifying growth characteristics, producing cytokines such as GM-CSF. Green Fluorescent Immortalized Human Aortic Endothelial Cells allow also to stablish in vitro models for HIgh Throughput Screening and High Content Screening.<\/p>\n<p>&nbsp;<\/p>\n<p><em><strong>About turboGFP protein:<\/strong><\/em><\/p>\n<p>tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation\/ emission max = 482\/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><strong>Green Fluorescent Immortalized Human Aortic Endothelial Cell Line<\/strong> (IM-HAEC-tGFP) provided by Innoprot has been developed by immortalizing primary human aortic endothelial cells with SV40 Large T antigen and transfected with tGFP.<\/p>\n<p>Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40\u2010tranduced cells go beyond 30 passages.<\/p>\n<p>Each vial of IM-HAEC-tGFP contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HAEC-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HAEC-tGFP culture.<\/p>\n","protected":false},"featured_media":7745,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[118],"product_tag":[],"class_list":["post-7744","product","type-product","status-publish","has-post-thumbnail","product_cat-fluorescent-immortalized-primary-cells","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Green Fluorescent Immortalized HAEC &#8212; Innoprot tGFP immortalized cells<\/title>\n<meta name=\"description\" content=\"Each vial of Green Fluorescent Immortalized Human Aortic Endothelial Cells contains more than 1 million cells, guaranteed to further culture\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/innoprot.com\/es\/producto\/green-fluorescent-immortalized-haec\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Green Fluorescent Immortalized HAEC &#8212; 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