{"id":7958,"date":"2023-02-12T00:00:21","date_gmt":"2023-02-11T23:00:21","guid":{"rendered":"http:\/\/innoprot.com\/?post_type=product&#038;p=7958"},"modified":"2023-02-12T00:00:22","modified_gmt":"2023-02-11T23:00:22","slug":"red-fluorescent-immortalized-human-colonic-fibroblasts","status":"publish","type":"product","link":"https:\/\/innoprot.com\/es\/producto\/red-fluorescent-immortalized-human-colonic-fibroblasts\/","title":{"rendered":"Red Fluorescent Immortalized Human Colonic Fibroblasts"},"content":{"rendered":"<p><em><strong>About HCoF:<\/strong><\/em><\/p>\n<p>Fibroblasts, are mesenchymal cells from the embryonic mesoderm. Fibroblasts secrete a non-rigid extracellular matrix that is both rich in type I and\/or type III collagen. They are responsible of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. They are useful tools for a wide range of cellular and molecular studies, as they are one of the easiest types of cells to grow in culture. In addition, their durability makes them amenable to a variety of manipulations ranging from gene transfection to microinjection. Red Fluorescent Immortalized Human Colonic Fibroblasts are an excellent tools to study the colonic physiology and also the mechanisms of colon carcinogenesis. Fluorescent Immortalized Human Colonic Fibroblasts are useful tools to understand the highly integrated balance between proliferation, differentiation, and cell death. This cell line also allow to stablish in vitro models for HIgh Throughput and High Content Screening..<\/p>\n<p>&nbsp;<\/p>\n<p><em><strong>About turboFP602 protein:<\/strong><\/em><\/p>\n<p>TurboFP602 protein is a red shifted variant of the red fluorescent protein TurboRFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. It possesses true-red fluorescence (excitation\/ emission max = 574\/ 602 nm) that is optimal for detection via most popular filter sets. TurboFP602 exhibits fast maturation and also high pH stability. TurboFP602 is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><strong>Red Fluorescent Immortalized Human Colonic Fibroblasts<\/strong> (IM-HCoF-tFP602) provided by Innoprot have been developed by immortalizing primary human colonic fibroblasts with SV40 Large T antigen and stably transfected with tFP602.<\/p>\n<p>Immortalized cells were controlled passaging side by side with the primary cells. These primary cells go into senescence after the 4th passage while the SV40\u2010tranduced cells go beyond 30 passages.<\/p>\n<p>Each vial of <strong>Red Fluorescent Immortalized Human Colonic Fibroblasts<\/strong> contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HCoF-tFP602 which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HCoF-tFP602 culture.<\/p>\n","protected":false},"featured_media":7959,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[118],"product_tag":[],"class_list":["post-7958","product","type-product","status-publish","has-post-thumbnail","product_cat-fluorescent-immortalized-primary-cells","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Red Fluorescent Immortalized Human Colonic Fibroblasts &#8212; Innoprot<\/title>\n<meta name=\"description\" content=\"Each vial of Red Fluorescent Immortalized Human Colonic Fibroblasts contains more than 1 million cells, guaranteed to further culture\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/innoprot.com\/es\/producto\/red-fluorescent-immortalized-human-colonic-fibroblasts\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Red Fluorescent Immortalized Human Colonic Fibroblasts &#8212; 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