{"id":843,"date":"2018-12-23T01:07:34","date_gmt":"2018-12-23T00:07:34","guid":{"rendered":"http:\/\/innoprot.com\/?post_type=product&#038;p=843"},"modified":"2026-01-13T10:07:06","modified_gmt":"2026-01-13T09:07:06","slug":"primary-cells-detach-kit","status":"publish","type":"product","link":"https:\/\/innoprot.com\/es\/producto\/primary-cells-detach-kit\/","title":{"rendered":"Primary Cells Detach Kit"},"content":{"rendered":"<p><strong>Primary Cells Detach Kit &#8211; Components Description:<\/strong><\/p>\n<p><strong>Dulbecco\u2019s Phosphate-Buffered Saline<\/strong> is a commonly used buffer in biological research. DPBS helps maintain a constant pH. The solution does not conatin Ca2+ or Mg2+ and is sterile-filtered. Because it is not toxic to cells and is isotonic, it may be used to rinse cells without causing any damage to them.<\/p>\n<p><strong>Trysin \/ EDTA solution<\/strong> (T\/E) is a sterile, phosphate and HEPES-buffered saline solution. It contains 0,25% trypsin, 0,5 mM EDTA, 1 mM sodium pyruvate and 10 mM HEPES. This product has a pH of 7.4 at room temperature. Trypsyn\/EDTA Solution is used to detach adherent cells from a culture surface.<\/p>\n<p><strong>Trysin Neutralization Solution<\/strong> (TNS) is a sterile, phosphate and HEPES-buffered saline solution. It contains 10% fetal bovine serum as a trypsin inhibitor and cell protect agents. The product is calcium- and magnesium-free and has a pH of 7.4 at room temperature. Trypsyn Neutralization Solution is used to neutralize the effects of Trypsin\/EDTA solution after the release of cells from a culture surface.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Primary Cells Detach Kit from Innoprot contains the following reagents for primary cells detach and subculture:<\/p>\n<ul>\n<li>500 ml DPBS (Dulbecco\u2019s Phosphate-Buffered Saline)<\/li>\n<li>100 ml T\/E Solution (Trysin \/ EDTA solution)<\/li>\n<li>100 ml TNS (Trysin Neutralization Solution)<\/li>\n<\/ul>\n","protected":false},"featured_media":844,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[70,74],"product_tag":[244,245],"class_list":["post-843","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-reagents","product_cat-subculturing-reagents","product_tag-detach-kit","product_tag-subculturing-kit","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Primary Cells Detach Kit &#8212; 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