Parkin mitochondrial recruitment assay cell line from Innoprot is based on localization changes of two fluorescent proteins; the Parkin fluorescent fusion polypeptide that changes its localization from the cytosol to the outer mitochondrial membrane (OMM); and the other fluorescent fusion polypeptide (MTS-tGFP), which binds directly to OMM, allowing the mitochondrial integrity analysis.<\/p>\n
Several compounds promote Parkin recruitment, but also generate stress and increase cell toxicity. So a good, promising positive compound would be one that promotes Parkin binding to mitochondria to accelerate Parkin protective function but without deeply altering mitochondrial integrity. Our model allows the identification of compounds that can promote the mitochondrial localization of Parkin without severe mitochondrial damage induction.<\/p>\n
Parkinson\u00b4s disease<\/strong><\/em> (PD) is a neurodegenerative disorder that originates with the death of dopamine producing cells in the substantia nigra in the brain. The main sympton is trembling in the hands, legs or face. Most cases of PD are sporadic, but 10% are autosomal dominant or recessive inheritance. Parkin is one of the proteins implicated in one form of familial Parkinson’s disease known as autosomal recessive juvenile Parkinson’s disease (AR-JP). It is a protein that forms part of E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. The recruitment of parkin by damaged mitochondria occurs during the early stages of mitrophagy.<\/p>\n Applications:<\/strong><\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" Parkin Mitochondrial Recruitment Assay Cell Line is a Parkinson’s Disease in vitro Model developed by Innoprot; which consists in U2OS cell line stably expressing the following constructs:<\/p>\n Innoprot offers this assay as a \u201cstable cell line\u201d in cryopreserved vials. Each vial of U2OS cell line stably expressing PARK2-FP602 &\u00a0MTS-tGFP contains 3 million cells and Innoprot provides 2 vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual. Size\/Quantity<\/strong>: 2 vials containing 3×106<\/sup> cells \/ vial<\/p>\n","protected":false},"featured_media":956,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[115],"product_tag":[283,282,307,894],"class_list":["post-955","product","type-product","status-publish","has-post-thumbnail","product_cat-stable-cell-lines-for-drug-screening-in-cns","product_tag-in-vitro-model","product_tag-parkin-recruitment","product_tag-parkinsons-disease","product_tag-pd-cell-lines","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"yoast_head":"\n\n
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