Scientific Reports

Article published: Assessing the role of surface glycans of extracellular vesicles on cellular uptake

Extracellular vesicles (EVs) are important mediators of cell-cell communication in a broad variety of physiological contexts. However, there is ambiguity around the fundamental mechanisms by which these effects are transduced, particularly in relation to their uptake by recipient cells. Multiple modes of cellular entry have been suggested and we have further explored the role of…

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Experimental Eye Research

Article published: Effect of somatostatin on human retinal pigment epithelial cells permeability

PURPOSE: To assess the effect of somatostatin (SST) on the permeability of human retinal pigment epithelial cells. METHODS: We conducted two experiments, exposing cells from human-fetal retinal pigment epithelium (hfRPE) cultures to vascular endothelial growth factor (VEGF), with or without SST pretreatment, in one, and to hypoxic conditions, again with or without SST pretreatment, in…

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Physiological Reports

Article published: Rescue of degenerating neurons and cells by stem cell released molecules: using a physiological renormalization strategy

Evidence suggests that adult stem cell types and progenitor cells act collectively in a given tissue to maintain and heal organs, such as muscle, through a release of a multitude of molecules packaged into exosomes from the different cell types. Using this principle for the development of bioinspired therapeutics that induces homeostatic renormalization, here we…

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Biomedicine and Pharmacotherapy

Synthesis, characterization, theoretical studies and biological (antioxidant, anticancer, toxicity and neuroprotective) determinations of a copper(II) complex with 5-hydroxytryptophan

5-Hydroxy-L-tryptophan (5-HTP) is a serotonin pathway metabolite of L-tryptophan in the brain. In the knowledge that the biological properties of some compounds can be modified upon metal complexation, a new solid metal complex, [Cu(5-hydroxytryptophan)2].H2O (Cu5HTP), has been synthesized and characterized to analyze the modification of some biological properties. The conformational investigations (optimized in gas phase…

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Meet us at Discovery on Target in Boston 25-28th September 2018

Innoprot will be participating as exhibitors and in the one-on-one partnering in the next “Discovery on Target” Conference, which will take place in Boston, MA, from the 25th to 28th, September, 2018. You can request a meeting with our delegates in the Convention, Dr. Jorge Gamiz, Business Development Director and Dr. Clarisa Salado, General Manager…

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What’s New At Innoprot – GPCR Nomad Cell Lines

A novel family of GPCR cell lines have been developed through stable transfection with different fluorescent Nomad reporter genes to monitor b-arrestin recruitment in combination with Ca++ increase measurement, allowing a multiplex funcitonal assay to characterize GPCR activation pathways and identify directly GPCR biased ligands. Each cell line is stably transfected with a label-free GPCR,…

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SLAS Technology

Nomad Biosensors: A New Multiplexed Technology for the Screening of GPCR Ligands

Nomad Technology (Innoprot [Innovative Technologies in Biological Systems], Derio, Spain), a novel tool for multiplexing high-throughput cell-based G protein-coupled receptor (GPCR) assays, is described in this work. This new technology comprises a family of fluorescent biosensors called Nomad Biosensors that allow for the measurement of responses mediated by G proteins through their interactions with second-messenger…

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PLOS Neglected Tropical Diseases

Inhibitors of Trypanosoma cruzi Sir2 related protein 1 as potential drugs against Chagas disease

Chagas disease remains one of the most neglected diseases in the world despite being the most important parasitic disease in Latin America. The characteristic chronic manifestation of chagasic cardiomyopathy is the region’s leading cause of heart-related illness, causing significant mortality and morbidity. Due to the limited available therapeutic options, new drugs are urgently needed to…

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