Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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NOMAD Adenosine A3 Receptor Cell Line

cAMPNOMAD Adenosine A3 Receptor Cell Line

9.995,00

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD Adenosine A3 Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human Adenosine A3 receptor (ADORA3) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD ADORA3 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to ADORA3, Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial