Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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α1A-adrenoceptor Cell Line

MPXNOMAD α1A-adrenoceptor Cell Line


MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD α1A-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human α1A-adrenoceptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD α1A-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1A a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.


Size/Quantity: 2 vials containing 3×106 cells / vial

HiTSeeker ADRA1A Cell Line

HiTSeeker alpha1A Adrenergic Receptor Cell Line


HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker alpha1A Adrenergic Receptor Cell Line contains U2OS cells stably expressing ADRA1A with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker ADRA1A Cell Line allows to assay compounds, or analyze their capability to modulate ADRA1A. When a ligand binds to the ADRA1A, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker ADRA1A cell line has been validated measuring calcium concentration changes in the cytosol upon activation with epinephrine. The high reproducibility of this assay allows monitoring ADRA1A receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial