Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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HiTSeeker BB2 Bombesin Receptor Cell LineCalcium Assay

HiTSeeker BB2 Bombesin Receptor Cell Line


HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker BB2 Bombesin Receptor Cell Line contains U2OS cells stably expressing BB2 Receptor with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker BB2 Bombesin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate BB2 receptor. When a ligand binds to the BB2 receptor, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker BB2 Receptor cell line has been validated measuring calcium concentration changes in the cytosol upon activation with GRP. The high reproducibility of this assay allows monitoring BB2 receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial