Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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Green Fluorescent Calcitonin Receptor

Green Fluorescent Calcitonin Receptor Cell Line

6.950,00€

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent Calcitonin Receptor Cell Line contains CHO-K1 cells stably expressing CALCR tagged with tGFP.  These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent Calcitonin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate CALCR activation and the following redistribution process inside the cells. When a ligand binds to the CALCR, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent CALCR redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring Calcitonin Receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

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HiTSeeker Calcitonin Receptor Cell Line

HiTSeeker Calcitonin Receptor Cell Line

5.250,00€

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker calcitonin receptor Cell Line contains HEK293 cells stably expressing CALCR with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker Calcitonin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate CALCR. When a ligand binds to the CALCR, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. HiTSeeker CALCR cell line has been validated measuring both, cAMP & Calcium concentration changes in the cytosol upon activation with calcitonin. The high reproducibility of this assay allows monitoring CALCR activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

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