Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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CB1 Cannabinoid Receptor Cell LineCB1 Cell Line

MPXNOMAD CB1 Cannabinoid Receptor Cell Line

19.950,00€

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD CB1 Cannabinoid Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human α1B-adrenoceptor with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)
  • β-arrestin Nomad Biosensor (Green Fluorescence)

MPXNOMAD CB1 Cannabinoid Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by cAMP flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to CB1 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and cAMP biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

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Fluorescent CB2 Cannabinoid Receptor

Green Fluorescent CB2 Cannabinoid Receptor Cell Line

6.950,00€

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent CB2 Cannabinoid Receptor Cell Line contains CHO-K1 cells stably expressing CB2 tagged with tGFP.  These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent CB2 Cannabinoid Receptor Cell Line allows to assay compounds, or analyze their capability to modulate CB2 activation and the following redistribution process inside the cells. When a ligand binds to the CB2 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent CB2 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring CB2 Receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

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CB1 Cannabinoid Receptor Cell LineCB1 Cannabinoid Receptor Cell Line

HiTSeeker CB1 Cannabinoid Receptor Cell Line

5.250,00€

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker CB1 Cannabinoid Receptor Cell Line contains HEK293 cells stably expressing CB1 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker CB1 Cell Line allows to assay compounds, or analyze their capability to modulate CB1. When a ligand binds to the CB1, it activates a G protein, which in turn, triggers a cellular response mediated by a second messenger. HiTSeeker CB1 cell line has been validated performing an assay with [3H]CP55940 and increasing concentrations of no radioactive competitor. The high reproducibility of this assay allows monitoring CB1 receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

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