Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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D1 Dopamine Receptor Cell Line

cAMPNOMAD D1 Dopamine Receptor Cell Line

9.995,00€

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD D1 Dopamine Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human D1 Dopamine Receptor (DRD1) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD DRD1 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to DRD1, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

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Fluorescent D1 Dopamine Receptor

Green Fluorescent D1 Dopamine Receptor Cell Line

6.950,00€

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent D1 Dopamine Receptor Cell Line contains SH-SY5Y cells stably expressing DRD1 Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent D1 Dopamine Receptor Cell Line allows to assay compounds, or analyze their capability to modulate DRD1 Receptor activation and the following redistribution process inside the cells. When a ligand binds to the DRD1 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent D1 Dopamine Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring DRD1 Receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

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HiTSeeker D1 Dopamine Receptor Cell Line

HiTSeeker D1 Dopamine Receptor Cell Line

5.250,00€

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker D1 Dopamine Receptor Cell Line contains HEK293 cells stably expressing DRD1 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker DRD1 Cell Line allows to assay compounds, or analyze their capability to modulate DRD1. When a ligand binds to the DRD1, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. HiTSeeker DRD1 cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with Dopamine. The high reproducibility of this assay allows monitoring DRD1 receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

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