Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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GLP-2 Receptor Cell Line

cAMPNOMAD GLP-2 Receptor Cell Line

9.995,00€

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD GLP-2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human GLP-2 Receptor (GLP2R) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD GLP-2 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to GLP-2, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

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Green Fluorescent GLP-2 Receptor

Green Fluorescent GLP-2 Receptor Cell Line

6.950,00€

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent GLP-2 Receptor Cell Line contains U2OS cells stably expressing GLP2R tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent GLP-2 Receptor Cell Line allows to assay compounds, or analyze their capability to modulate GLP2R activation and the following redistribution process inside the cells. When a ligand binds to the GLP-2 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent GLP2R redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring GLP-2 Receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

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