Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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H1 Histamine ReceptorHRH1 Cell Line

MPXNOMAD H1 Histamine Receptor Cell Line

19.950,00€

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD H1 Histamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human H1 Histamine Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD H1 Histamine Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to H1 receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

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HiTSeeker Histamine H1 Receptor Cell LineCalcium Assay

HiTSeeker Histamine H1 Receptor Cell Line

5.250,00€

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker Histamine H1 Receptor Cell Line contains U2OS cells stably expressing H1 Receptor with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker Histamine H1 Receptor Cell Line allows to assay compounds, or analyze their capability to modulate H1 receptor. When a ligand binds to the BB2 receptor, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker H1 Receptor cell line has been validated measuring calcium concentration changes in the cytosol upon activation with Histamine. The high reproducibility of this assay allows monitoring H1 Receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

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