Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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Muscarinic M5 Receptor Cell Line

DAGNOMAD Muscarinic Acetylcholine Receptor M5 Cell Line

9.995,00€

DAGNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and diacylglycerol (DAG) Nomad Biosensor to monitor G-protein modulation by DAG release. Each vial of DAGNOMAD Muscarinic M5 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human Muscarinic M5 Receptor (M5R) with no tag
  • DAG Nomad Biosensor (Red Fluorescence)

cAMPNOMAD Muscarinic M5 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gq/G11 involving receptor activation. When an agonist binds to M5 Receptor, the Gq/G11 protein is activated which, in turn, triggers a cellular response mediated by DAG via Phospholipase C stimulation. This leads to an increase of red fluorescence intensity of DAG biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

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HiTSeeker Muscarinic Acetylcholine Receptor M5 Cell LineHiTSeeker Vasopressin Receptor 1A Cell Line

HiTSeeker Muscarinic Acetylcholine Receptor M5 Cell Line

5.250,00€

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker Muscarinic Acetylcholine Receptor M5 Cell Line contains U2OS cells stably expressing CHRM5 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker Muscarinic Acetylcholine Receptor M5 Cell Line allows to assay compounds, or analyze their capability to modulate CHRM5. When a ligand binds to the CHRM5, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker CHRM5 cell line has been validated measuring calcium concentration changes in the cytosol upon activation with oxotremorine. The high reproducibility of this assay allows monitoring CHRM5 receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

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