Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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NOMAD PAR2 Receptor Cell LineNomad PAR2 Cell Line

MPXNOMAD PAR2 Receptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD PAR2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human PAR2 Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD PAR2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to PAR2 Receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial