Innoprot offers different cell lines stably expressing GPCRs to monitor their activation through different pathways:

  • HiTSeeker Cell Lines express GPCR with no tag
  • Internalization Cell Lines express GPCR tagged to tGFP to monitor internalization assays.
  • Nomad Cell Lines co-express GPCR with no tag and Nomad Biosensors to monitor simultaneously the different GPCR signalling pathways (β-arrestin recruitment, Ca2+, cAMP or also DAG).

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NOMAD VPAC1 Receptor Cell Line

cAMPNOMAD VPAC1 Receptor Cell Line

9.995,00

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD VPAC1 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human VIP Receptor 1 (VPAC1R) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD VPAC1 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to PACAP-38, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

VPAC1 Receptor Cell Line

HiTSeeker VPAC1 Receptor Cell Line

5.250,00

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker VPAC1 Receptor Cell Line contains HEK293 cells stably expressing vasoactive intestinal peptide receptor 1 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker VPAC1 Receptor Cell Line allows to assay compounds, or analyze their capability to modulate VPAC1R. When a ligand binds to the VIPR1, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. HiTSeeker VPAC1 Receptor Cell Line has been validated measuring cAMP concentration changes in the cytosol upon activation with PACAP-38. The high reproducibility of this assay allows monitoring VPAC1 Receptor activation process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial