Innoprot assay development platform combinens both target-based and phenotypic screening due to their important role to move from in-vitro to in-vivo pharmacology for a high range of disease pathways. It can be directed toward the best detection technology for a given molecular target and screening objective.

Innoprot offers in vitro models for many diseases, including Alzheimer’s Disease, Parkinson’s Disease, Amyotrophic Laterial Sclerosis or Ischemic Stroke. These models mimick known pathways associated to each disease, allowing the screening of compounds of these diseases directly using of fluorescent microscopy analysis.

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APP Processing Assay Cell LineAPP Processing Assay Cell Line

APP Processing Assay Cell Line

9.995,00€

APP Processing Assay Cell Line is an Alzheimer’s Disease in vitro model to identify Aβ peptide formation inhibitors such as secretase activity inhibitors. It consists in MDCK cell line stably expressing green fluorescent human amyloid precursor protein (APP). To perform the assay, cells are incubated with test compounds during 48-72 hours to evaluate their effects on APP Processing. After incubation, nucleus could be stained with DAPI and retained fluorescent APP vesicles are detected by fluorescence using image analysis algorithms.

Innoprot offers this assay as a “stable cell line” in cryopreserved vials. Each vial of MDCK cell line stably expressing human tGFP-APP contains 3 million cells and Innoprot provides 2 vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

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FUS/TLS Stress Granules Assay Cell LineFUS/TLS Stress Granules Assay Cell Line

FUS/TLS Stress Granules Assay Cell Line

9.995,00€

Innoprot has developed a novel fluorescence cell-based assay cell line for High Content Screening that allows the quantification of FUS/TLS stress granules. This model consists in a U2OS cell line stably expressing fluorescent RNA-binding protein FUS/TLS (Fused in Sarcoma/Translocated in Sarcoma).

Innoprot offers this cell line as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing fluorescent FUS/TLS contains 3 million cells and Innoprot provides 2 vials of cells with each order. These cells proliferate in vitro and they are guaranteed to further expand for more than 30 passages following the instructions provided in the technical sheet.

Size/Quantity: 2 vials containing 3×106 cells / vial

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Gamma Secretase Activity Assay Cell LineGamma Secretase Activity Assay Cell Line

Gamma Secretase Activity Assay Cell Line

9.995,00€

Gamma Secretase Activity Assay Cell Line is an Alzheimer’s Disease in vitro model to identify γ-secretase activity inhibitors. It consists in U2OS cell line stably expressing green fluorescent APP-C99 construct, which contains the γ-secretase cleaving site. To perform the assay, cells are incubated with test compounds during 24 hours to evaluate their effects on APP Processing. After incubation, nucleus could be stained with DAPI and retained fluorescent APP vesicles are detected by fluorescence using image analysis algorithms.

Innoprot offers this assay as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing human tGFP-APP-C99 contains 3 million cells and Innoprot provides 2 vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

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Neurite Outgrowth & Mitosis Assay Cell LineNeurite Outgrowth & Mitosis Assay Cell Line

Neurite Outgrowth & Mitosis Assay Cell Line

1.950,00€

Neurite Outgrowth & Mitosis Assay Cell Line (TagGFP2‐Tubulin SH‐SY5Y Cell line) allows the analysis of neurites while monitoring the cellular mitosis level through microtubule structural changes. It consists in SH-SY5Y cell line stably expressing green fluorescent tubulin marking the cell cytoskeleton and it can be easly differentiated using standardized protocols to promote the neurite outgrowth.

Innoprot offers this assay as a “stable cell line” in cryopreserved vials. Each vial of SH-SY5Y cell line stably expressing TagGFP2-tubulin contains 3 million cells. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

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Parkin Mitochondrial Recruitment Assay Cell LineParkin Mitochondrial Recruitment Assay Cell Line

Parkin Mitochondrial Recruitment Assay Cell Line

9.995,00€

Parkin Mitochondrial Recruitment Assay Cell Line is a Parkinson’s Disease in vitro Model developed by Innoprot; which consists in U2OS cell line stably expressing the following constructs:

  • Green fluorescent protein (tGFP) fused to mitochondrial targeting sequence (MTS) derived from the subunit VIII of human cytochrome C oxidase
  • PARK2 protein fused to FP602 red fluorescent protein.

Innoprot offers this assay as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing PARK2-FP602 & MTS-tGFP contains 3 million cells and Innoprot provides 2 vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.
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Size/Quantity: 2 vials containing 3×106 cells / vial

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SOD1 A4V Aggregation Assay Cell Line

SOD1 A4V Aggregation Assay Cell Line

6.995,00€

Innoprot has developed a novel fluorescence cell-based assay cell line for High Content Screening that allows the quantification of SOD1 aggregation on living cells. This model consists in a HEK293 cell line stably expressing red fluorescent A4V mutant of SOD1. This cell line has been designed to assay compounds or analyze their capability to modulate superoxide dismutase 1 tendency to form aggregates inside the cell. The addition of proteasome inhibitor ALLN, produces the aggregation of the protein in HEK293 cells. Co-incubation with test-compounds, allows the identification of potential inhibitors of SOD1 aggregation.

Innoprot offers this cell line as a “stable cell line” in cryopreserved vials. Each vial of HEK293 cell line stably expressing red fluorescent tagged (TurboFP602) SOD1-A4V protein contains 3 million cells and Innoprot provides 2 vials of cells with each order. These cells proliferate in vitro and they are guaranteed to further expand for more than 30 passages following the instructions provided in the technical sheet.

Size/Quantity: 2 vials containing 3×106 cells / vial

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tau phosphorylation assay cell linetau phosphorylation assay cell line

tau Phosphorylation Assay Cell Line

9.995,00€

tau Phosphorilation Assay Cell Line is an Alzheimer’s Disease in vitro model to identify tau kinase and phosphatase inhibitors and modulators. It consists in U2OS cell line stably expressing human triple mutant Tau-tGFP. To perform the assay, cells are incubated with test compounds during 2 hours to evaluate their effects on Tau phosphorylation and binding to microtubules. After incubation, nucleus could be stained with DAPI and cells Tau and MT bundles are detected by fluorescence using image analysis algorithms.

Innoprot offers this assay as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing human triple mutant Tau-tGFP contains 3 million cells and Innoprot provides 2 vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

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TDP-43 Stress Granules Assay Cell LineTDP-43 Stress Granules Assay Cell Line

TDP-43 Stress Granules Assay Cell Line

9.995,00€

Innoprot has developed a novel fluorescence cell-based assay cell line for High Content Screening that allows the quantification of pathological TDP43 globs into the nucleus and cytosol. This model consists in a U2OS cell line stably expressing inducible fluorescent TDP-43. U2O2 cells stably expressing human TAR DNA-binding protein 43 (TDP-43) must be induced with IPTG 5mM during 48h to produce the hTDP43-tGFP protein.

Innoprot offers this cell line as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing fluorescent TDP-43 contains 3 million cells and Innoprot provides 2 vials of cells with each order. These cells proliferate in vitro and they are guaranteed to further expand for more than 30 passages following the instructions provided in the technical sheet.

Size/Quantity: 2 vials containing 3×106 cells / vial

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TDP43-FUS Cell Line

TDP43-FUS Cell Line

9.995,00€

Innoprot has developed a novel fluorescence cell-based assay cell line for High Content Screening that allows the quantification of both TDP-43 and FUS/TLS stress granules. This model consists in a U2OS cell line stably expressing green fluorescent TDP-43 and red fluorescent FUS/TLS.

Innoprot offers this cell line as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing green fluorescent TDP-43 and red fluorescent FUS/TLS contains 3 million cells and Innoprot provides 2 vials of cells with each order. These cells proliferate in vitro and they are guaranteed to further expand for more than 30 passages following the instructions provided in the technical sheet.

Size/Quantity: 2 vials containing 3×106 cells / vial

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