Showing all 7 results

α1A-adrenoceptor Cell Line

MPXNOMAD α1A-adrenoceptor Cell Line

19.950,00€

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD α1A-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human α1A-adrenoceptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD α1A-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1A a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

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α1B-adrenoceptor Cell Lineα1B-adrenoceptor Cell Line

MPXNOMAD α1B-adrenoceptor Cell Line

19.950,00€

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD α1B-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human α1B-adrenoceptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD α1B-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1B a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

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Green Fluorescent beta-2 adrenoreceptor

Green Fluorescent beta-2 adrenoreceptor Cell Line

6.950,00€

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent beta-2 adrenoreceptor Cell Line contains CHO-K1 cells stably expressing ADRB2 tagged with tGFP.  These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent beta-2 adrenoreceptor Cell Line allows to assay compounds, or analyze their capability to modulate ADRB2 activation and the following redistribution process inside the cells. When a ligand binds to the ADRB2, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent ADRB2 redistribution by image analysis as an increment of fluorescent intensity of these vesicles. The high reproducibility of this assay allows monitoring ADRB2 receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

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HiTSeeker ADRA1A Cell Line

HiTSeeker alpha1A Adrenergic Receptor Cell Line

5.250,00€

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker alpha1A Adrenergic Receptor Cell Line contains U2OS cells stably expressing ADRA1A with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker ADRA1A Cell Line allows to assay compounds, or analyze their capability to modulate ADRA1A. When a ligand binds to the ADRA1A, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker ADRA1A cell line has been validated measuring calcium concentration changes in the cytosol upon activation with epinephrine. The high reproducibility of this assay allows monitoring ADRA1A receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

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HiTSeeker alpha1B Adrenergic Receptor Cell Line

HiTSeeker alpha1B Adrenergic Receptor Cell Line

5.250,00€

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker alpha1B Adrenergic Receptor Cell Line contains U2OS cells stably expressing ADRA1B with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker ADRA1B Cell Line allows to assay compounds, or analyze their capability to modulate ADRA1B. When a ligand binds to the ADRA1B, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker ADRA1B cell line has been validated measuring calcium concentration changes in the cytosol upon activation with epinephrine. The high reproducibility of this assay allows monitoring ADRA1B receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

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HiTSeeker beta-2 adrenergic receptor Cell Line

HiTSeeker beta-2 adrenergic receptor Cell Line

5.250,00€

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker beta-2 adrenergic receptor Cell Line contains HEK293 cells stably expressing ADRB2 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker ADRB2 Cell Line allows to assay compounds, or analyze their capability to modulate ADRB2. When a ligand binds to the ADRB2, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. HiTSeeker ADRB2 cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with Isoproterenol. The high reproducibility of this assay allows monitoring ADRB2 receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

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HiTSeeker beta-3 adrenergic receptor Cell Line

HiTSeeker beta-3 adrenergic receptor Cell Line

5.250,00€

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker beta-3 adrenergic receptor Cell Line contains HEK293 cells stably expressing ADRB3 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker ADRB3 Cell Line allows to assay compounds, or analyze their capability to modulate ADRB3. When a ligand binds to the ADRB3, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. HiTSeeker ADRB3 cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with isoproterenol. The high reproducibility of this assay allows monitoring ADRB3 receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial

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