cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD ADRB2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
- human B2 adrenoceptor with no tag
- cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD B2 adrenoceptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by cAMP involving receptor activation. When an agonist binds to ADRB2 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
Size/Quantity: 2 vials containing 3×106 cells / vial