Innoprot has developed a novel fluorescence cell-based assay cell line for High Content Screening that allows the quantification of pathological TDP43 globs into the nucleus and cytosol. This model consists in a U2OS cell line stably expressing inducible fluorescent TDP-43. U2O2 cells stably expressing human TAR DNA-binding protein 43 (TDP-43) must be induced with IPTG 5mM during 48h to produce the hTDP43-tGFP protein.
Innoprot offers this cell line as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing fluorescent TDP-43 contains 3 million cells and Innoprot provides 2 vials of cells with each order. These cells proliferate in vitro and they are guaranteed to further expand for more than 30 passages following the instructions provided in the technical sheet.
Size/Quantity: 2 vials containing 3×106 cells / vial