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Gamma Secretase Activity Assay Cell LineGamma Secretase Activity Assay Cell Line

Gamma Secretase Activity Assay Cell Line

9.995,00€

Gamma Secretase Activity Assay Cell Line is an Alzheimer’s Disease in vitro model to identify γ-secretase activity inhibitors. It consists in U2OS cell line stably expressing green fluorescent APP-C99 construct, which contains the γ-secretase cleaving site. To perform the assay, cells are incubated with test compounds during 24 hours to evaluate their effects on APP Processing. After incubation, nucleus could be stained with DAPI and retained fluorescent APP vesicles are detected by fluorescence using image analysis algorithms.

Innoprot offers this assay as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing human tGFP-APP-C99 contains 3 million cells and Innoprot provides 2 vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

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Green Fluorescent BACE1 Cell Line

Green Fluorescent BACE1 Cell Line

1.995,00€

A novel family of stable cell lines have been developed through stable transfection with green fluorescent BACE1. Green Fluorescent BACE1 Cell Lines are stably-transfected and it is ready to use in cell-based assay applications. These stably transfected cell linew provide consistent levels of expression, which helps to simplify the interpretation of the results. These cell lines are intended to be used as an “in vitro” models for research studies.

 

Cell Lines available

  • SH-SY5Y cells expressing fluorescent BACE1
  • HEK293 cells expressing fluorescent BACE1
  • U2OS cells expressing fluorescent BACE1

All vials of the different stable cell line contains 3 million cells. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual

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Green fluorescent tau SH-SY5Y cell line

Green fluorescent tau SH-SY5Y cell line

2.950,00€

A novel family of SH-SY5Y cell lines have been developed through stable transfection with different fluorescent isoforms of tau protein and mutants.

Each cell line is stably‐transfected and it is ready to use in cell‐based assay applications. These stably transfected cell lines provide consistent levels of expression, which helps to simplify the interpretation of the results. These cell lines are intended to be used as “in vitro” models for tau research studies.

 

Cell Lines available

  • SH-SY5Y cells expressing tau-tGFP (0N4R isoform)
  • SH-SY5Y cells expressing tau-tGFP (0N3R isoform)
  • SH-SY5Y cells expressing Tau-tGFP (0N4R (G272V mutant))
  • SH-SY5Y cells expressing Tau-tGFP (0N4R(^K280 mutant))
  • SH-SY5Y cells expressing Tau-tGFP (0N4R(P301L mutant))
  • SH-SY5Y cells expressing Tau-tGFP (0N4R(G272V-^K280 mutant))
  • SH-SY5Y cells expressing Tau-tGFP (0N4R(G272V-P301L mutant))
  • SH-SY5Y cells expressing Tau-tGFP (0N4R(^K280-P301L mutant))
  • SH-SY5Y cells expressing Tau-tGFP (0N4R(G272V-^K280-P301L mutant))

Each vial of cells contains 3 million cells It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

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SH-SY5Y stably expressing tau-tGFP & alpha-synuclein-TagRFPSH-SY5Y stably expressing tau-tGFP & alpha-synuclein-TagRFP

SH-SY5Y stably expressing tau-tGFP & alpha-synuclein-TagRFP

3.950,00€

A novel SH‐SY5Y cell line has been developed through stable transfection with tGPF‐tau protein (0N4R isoform-G272V mutation) and TagRFP‐α‐synuclein (wild type). This cell line is stably‐transfected with both construcst and it is ready to use in cell‐based assay applications. This stably transfected cell line provides consistent levels of expression, which helps to simplify the interpretation of the results. This cell line is intended to be used as an “in vitro” model for research studies.

Each vial of this cell line stably expressing human tau(0N4R-G272V)-tGFP and TagRFP‐α‐synuclein contains 3 million cells. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

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tau phosphorylation assay cell linetau phosphorylation assay cell line

tau Phosphorylation Assay Cell Line

9.995,00€

tau Phosphorilation Assay Cell Line is an Alzheimer’s Disease in vitro model to identify tau kinase and phosphatase inhibitors and modulators. It consists in U2OS cell line stably expressing human triple mutant Tau-tGFP. To perform the assay, cells are incubated with test compounds during 2 hours to evaluate their effects on Tau phosphorylation and binding to microtubules. After incubation, nucleus could be stained with DAPI and cells Tau and MT bundles are detected by fluorescence using image analysis algorithms.

Innoprot offers this assay as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing human triple mutant Tau-tGFP contains 3 million cells and Innoprot provides 2 vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.

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