MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD H1 Histamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human H1 Histamine Receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD H1 Histamine Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to H1 receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor both G-protein pathways. Each vial of MPXNOMAD NK2 Tachykinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human NK2 Receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
cAMP Nomad Biosensor (Red Fluorescence)
MPXNOMAD NK2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; both G proteins by Ca2+ & cAMP flux, hence allowing biased activity studies. When an agonist binds to NK2 Receptor two G proteins are activated which, in turn, triggers a cellular response mediated by calcium and cAMP. This leads to an increase of fluorescence intensity of both cAMP and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD NTS1 Neurotensin Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human NTS1 Neurotensin Receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD NTS1 Neurotensin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to NTS1R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD PAR2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human PAR2 Receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD PAR2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to PAR2 Receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD α1A-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs:
human α1A-adrenoceptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD α1A-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1A a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD α1B-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs:
human α1B-adrenoceptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD α1B-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1B a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
PKANOMAD Cells are cell lines stably co-expressing tag-free GPCRs and PKA Nomad Biosensor to monitor G-protein modulation by PKA activation. Each vial of PKANOMAD Muscarinic M4 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human Muscarinic M4 Receptor (M4R) with no tag
PKA Nomad Biosensor (Red Fluorescence)
PKANOMAD Muscarinic M4 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gi/Go involving receptor activation. When an agonist binds to M4 Receptor, the Gi/Go protein is activated which, in turn, triggers a cellular response mediated by cAMP via PKA activation. This leads to an increase of red fluorescence intensity of PKA biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
β-ArNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and β-arrestin Nomad Biosensor to GPCR β-arrestin modulation. Each vial of β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human CCK2 Cholecystokinin Receptor with no tag
β-arrestin Nomad Biosensor (Green Fluorescence)
β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line allows to assay compounds analyzing β-arrestin recruitment. When an agonist binds to CCK2R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. Due to the presence of ß-Arrestin Nomad Biosensor, this recruitment leads to an increase of green fluorescence intensity. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
FluoHiTSeeker are cell lines stably co-expressing label-free GPCRs and the CRE-tGFP reporter gene. Each vial of FluoHiTSeeker FSHR Cell Line contains HEK293 cells stably expressing human Follicle Stimulating Hormone Receptor with no tag; and the CRE-tGFP reporter gene as a biosensor to monitor cAMP concentration changes. FluoHiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.
Innoprot FluoHiTSeeker FSHR Cell Line allows to assay compounds, or analyze their capability to modulate FSHR. When a ligand binds to the FSHR, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. Due to the presence of the CRE-tGFP reporter gene, this cell line allows to monitor cAMP concentration in living cells measuring fluorescence intensity. FluoHiTSeeker FSHR cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with rhFSH. The high reproducibility of this assay allows monitoring FSH receptor activation process in High Throughput Screening.
FluoHiTSeeker are cell lines stably co-expressing label-free GPCRs and the CRE-tGFP reporter gene. Each vial of FluoHiTSeeker LHCGR Cell Line contains HEK293 cells stably expressing human Luteinizing Hormone/Choriogonadotropin Receptor with no tag; and the CRE-tGFP reporter gene as a biosensor to monitor cAMP concentration changes. FluoHiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.
Innoprot FluoHiTSeeker LHCGR Cell Line allows to assay compounds, or analyze their capability to modulate LHCGR. When a ligand binds to the LHCGR, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. Due to the presence of the CRE-tGFP reporter gene, this cell line allows to monitor cAMP concentration in living cells measuring fluorescence intensity. FluoHiTSeeker LHCGR cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with rhCG. The high reproducibility of this assay allows monitoring FSH receptor activation process in High Throughput Screening.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent B1 Bradykinin Receptor Cell Line contains CHO-K1 cells stably expressing BDKRB1 tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent B1 Bradykinin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate BDKRB1 activation and the following redistribution process inside the cells. When a ligand binds to the BDKRB1, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent BDKRB1 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring B1 Bradykinin Receptor redistribution process in High Throughput Screening.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent B2 Bradykinin Receptor Cell Line contains CHO-K1 cells stably expressing BDKRB2 tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent B2 Bradykinin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate BDKRB2 activation and the following redistribution process inside the cells. When a ligand binds to the BDKRB2, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent BDKRB2 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring B2 Bradykinin Receptor redistribution process in High Throughput Screening.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent beta-2 adrenoreceptor Cell Line contains CHO-K1 cells stably expressing ADRB2 tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent beta-2 adrenoreceptor Cell Line allows to assay compounds, or analyze their capability to modulate ADRB2 activation and the following redistribution process inside the cells. When a ligand binds to the ADRB2, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent ADRB2 redistribution by image analysis as an increment of fluorescent intensity of these vesicles. The high reproducibility of this assay allows monitoring ADRB2 receptor redistribution process in High Throughput Screening.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent Calcitonin Receptor Cell Line contains CHO-K1 cells stably expressing CALCR tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent Calcitonin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate CALCR activation and the following redistribution process inside the cells. When a ligand binds to the CALCR, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent CALCR redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring Calcitonin Receptor redistribution process in High Throughput Screening.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent CB2 Cannabinoid Receptor Cell Line contains CHO-K1 cells stably expressing CB2 tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent CB2 Cannabinoid Receptor Cell Line allows to assay compounds, or analyze their capability to modulate CB2 activation and the following redistribution process inside the cells. When a ligand binds to the CB2 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent CB2 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring CB2 Receptor redistribution process in High Throughput Screening.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent CCR2 Chemokine Receptor Cell Line contains U2OS cells stably expressing CCR2 tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent CCR2 Chemokine Receptor Cell Line allows to assay compounds, or analyze their capability to modulate CCR2 activation and the following redistribution process inside the cells. When a ligand binds to the CCR2 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent CCR2 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring CCR2 Receptor redistribution process in High Throughput Screening.
Green Fluorescent Calcitonin Receptor Cell Line 
