DAGNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and diacylglycerol (DAG) Nomad Biosensor to monitor G-protein modulation by DAG release. Each vial of DAGNOMAD Muscarinic M5 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
- human Muscarinic M5 Receptor (M5R) with no tag
- DAG Nomad Biosensor (Red Fluorescence)
cAMPNOMAD Muscarinic M5 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gq/G11 involving receptor activation. When an agonist binds to M5 Receptor, the Gq/G11 protein is activated which, in turn, triggers a cellular response mediated by DAG via Phospholipase C stimulation. This leads to an increase of red fluorescence intensity of DAG biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
Size/Quantity: 2 vials containing 3×106 cells / vial