cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD Adenosine A1 Receptor Cell Line contains HEK293 cells stably expressing the following constructs:
human Adenosine A1 receptor (ADORA1) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD ADORA1 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to ADORA1, Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD Adenosine A3 Receptor Cell Line contains HEK293 cells stably expressing the following constructs:
human Adenosine A3 receptor (ADORA3) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD ADORA3 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to ADORA3, Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD ADRB2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human B2 adrenoceptor with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD B2 adrenoceptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by cAMP involving receptor activation. When an agonist binds to ADRB2 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD ADRB3 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human B3 adrenoceptor with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD B3 adrenoceptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by cAMP involving receptor activation. When an agonist binds to ADRB3 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD AVPR2 Cell Line contains U2OS cells stably expressing the following constructs:
human AVPR2 with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD AVPR2 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to [Arg8]‐AVP, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD D1 Dopamine Receptor Cell Line contains HEK293 cells stably expressing the following constructs:
human D1 Dopamine Receptor (DRD1) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD DRD1 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to DRD1, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD D2 Dopamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human D2 Dopamine Receptor (DRD2) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD DRD2 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to DRD2, the Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD D5 Dopamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human D5 Dopamine Receptor (DRD5) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD DRD5 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to DRD5, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD GIP Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human GIP Receptor (GIPR) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD GIP Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to GIP, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD GLP-1 Receptor Cell Line contains HEK293 cells stably expressing the following constructs:
human GLP-1 Receptor (GLP1R) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD GLP-1 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to GLP-1, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD GLP-2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human GLP-2 Receptor (GLP2R) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD GLP-2 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to GLP-2, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD VPAC1 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human VIP Receptor 1 (VPAC1R) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD VPAC1 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to PACAP-38, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD VPAC2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human VIP Receptor 2 (VPAC2R) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD VPAC2 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to PACAP-38, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
DAGNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and diacylglycerol (DAG) Nomad Biosensor to monitor G-protein modulation by DAG release. Each vial of DAGNOMAD Muscarinic M5 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human Muscarinic M5 Receptor (M5R) with no tag
DAG Nomad Biosensor (Red Fluorescence)
cAMPNOMAD Muscarinic M5 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gq/G11 involving receptor activation. When an agonist binds to M5 Receptor, the Gq/G11 protein is activated which, in turn, triggers a cellular response mediated by DAG via Phospholipase C stimulation. This leads to an increase of red fluorescence intensity of DAG biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD CB1 Cannabinoid Receptor Cell Line contains HEK293 cells stably expressing the following constructs:
human α1B-adrenoceptor with no tag
cAMP Nomad Biosensor (Red Fluorescence)
β-arrestin Nomad Biosensor (Green Fluorescence)
MPXNOMAD CB1 Cannabinoid Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by cAMP flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to CB1 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and cAMP biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human CCK2 Cholecystokinin Receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to CCK2R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
