Showing 17–24 of 24 results

H1 Histamine ReceptorHRH1 Cell Line

MPXNOMAD H1 Histamine Receptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD H1 Histamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human H1 Histamine Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD H1 Histamine Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to H1 receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

NOMAD NK2 Tachykinin Receptor Cell LineNK2R

MPXNOMAD NK2 Tachykinin Receptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor both G-protein pathways. Each vial of MPXNOMAD NK2 Tachykinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human NK2 Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • cAMP Nomad Biosensor (Red Fluorescence)

MPXNOMAD NK2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; both G proteins by Ca2+ & cAMP flux, hence allowing biased activity studies. When an agonist binds to NK2 Receptor two G proteins are activated which, in turn, triggers a cellular response mediated by calcium and cAMP. This leads to an increase of fluorescence intensity of both cAMP and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

NTS1 Neurotensin Receptor Cell Line

MPXNOMAD NTS1 Neurotensin Receptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD NTS1 Neurotensin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human NTS1 Neurotensin Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD NTS1 Neurotensin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to NTS1R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

NOMAD PAR2 Receptor Cell LineNomad PAR2 Cell Line

MPXNOMAD PAR2 Receptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD PAR2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human PAR2 Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD PAR2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to PAR2 Receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

α1A-adrenoceptor Cell Line

MPXNOMAD α1A-adrenoceptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD α1A-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human α1A-adrenoceptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD α1A-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1A a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

α1B-adrenoceptor Cell Lineα1B-adrenoceptor Cell Line

MPXNOMAD α1B-adrenoceptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD α1B-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human α1B-adrenoceptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD α1B-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1B a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

M4 Muscarinic Receptor Cell Line

PKANOMAD Muscarinic Acetylcholine Receptor M4 Cell Line

9.995,00

PKANOMAD Cells are cell lines stably co-expressing tag-free GPCRs and PKA Nomad Biosensor to monitor G-protein modulation by PKA activation. Each vial of PKANOMAD Muscarinic M4 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human Muscarinic M4 Receptor (M4R) with no tag
  • PKA Nomad Biosensor (Red Fluorescence)

PKANOMAD Muscarinic M4 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gi/Go involving receptor activation. When an agonist binds to M4 Receptor, the Gi/Go protein is activated which, in turn, triggers a cellular response mediated by cAMP via PKA activation. This leads to an increase of red fluorescence intensity of PKA biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Lineβ-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line

β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line

9.995,00

β-ArNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and β-arrestin Nomad Biosensor to GPCR β-arrestin modulation. Each vial of β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human CCK2 Cholecystokinin Receptor with no tag
  • β-arrestin Nomad Biosensor (Green Fluorescence)

β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line allows to assay compounds analyzing β-arrestin recruitment. When an agonist binds to CCK2R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. Due to the presence of ß-Arrestin Nomad Biosensor, this recruitment leads to an increase of green fluorescence intensity. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial