MPXNOMAD NK2 Tachykinin Receptor Cell Line
19.950,00€MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor both G-protein pathways. Each vial of MPXNOMAD NK2 Tachykinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:
- human NK2 Receptor with no tag
- Calcium Nomad Biosensor (Green Fluorescence)
- cAMP Nomad Biosensor (Red Fluorescence)
MPXNOMAD NK2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; both G proteins by Ca2+ & cAMP flux, hence allowing biased activity studies. When an agonist binds to NK2 Receptor two G proteins are activated which, in turn, triggers a cellular response mediated by calcium and cAMP. This leads to an increase of fluorescence intensity of both cAMP and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
Size/Quantity: 2 vials containing 3×106 cells / vial