cAMPNOMAD Adenosine A1 Receptor Cell Line

Reference: P70523

cAMPNOMAD Adenosine A1 Receptor Cell Line

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD Adenosine A1 Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human Adenosine A1 receptor (ADORA1) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD ADORA1 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to ADORA1, Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

9.995,00

Related Products:

You may also like…

The adenosine A1 receptor (ADORA1) is one member of the adenosine receptor group of GPCRs with adenosine as endogenous ligand. Activation of the adenosine A1 receptor by an agonist causes binding of Gi1/2/3 or Go protein. Binding of Gi1/2/3 causes an inhibition of adenylate cyclase and, therefore, a decrease in the cAMP concentration.

The adenosine A1 receptor appears throughout the entire body and plays an important role in sleep promotion by inhibiting wake-promoting cholinergic neurons in the basal forebrain. A1 receptors are also present in smooth muscle throughout the vascular system.

Click HERE to know more about ADORA1 Receptor.

 

  • Reference: P70523
  • Nomad Biosensors: cAMP
  • GeneBank Accesion Number: NM_000674
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice