_cAMPNOMAD FSHR Cell Line

Reference: P70512

9.995,00€

_cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of _cAMPNOMAD FSHR Cell Line contains U2OS cells stably expressing the following constructs:

Human Follicle stimulating hormone receptor (FSHR) with no tag
cAMP Nomad Biosensor (Red Fluorescence)

_cAMPNOMAD FSHR Cell Line allows for the assay of compounds analyzing the G-Protein signalling pathway via cAMP, following activation of the receptor. When an agonist binds to FSHR, a G protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase in the red fluorescence intensity of cAMP biosensor. Thus, the activity can be easily quantified in living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial

The follicle-stimulating hormone receptor (FSHR) is a G protein-coupled receptor (GPCR) involved in the regulation of reproductive processes. It binds follicle-stimulating hormone (FSH), a glycoprotein hormone that activates Gs proteins. This leads to adenylate cyclase stimulation and increased intracellular cAMP levels. This signaling pathway is essential for ovarian follicle development and spermatogenesis. _cAMPNomad FSHR Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as FSH.

Red Fluorescent cAMP Nomad Biosensor also expressed in the cell line, allows monitoring cAMP concentration changes when receptor is activated/inhibited.

Click HERE to know more about FSH Receptor.