cAMPNOMAD GIP Receptor Cell Line

Reference: P70529

cAMPNOMAD GIP Receptor Cell Line

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD GIP Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human GIP Receptor (GIPR) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD GIP Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to GIP, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

9.995,00

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The Gastric inhibitory polypeptide receptor (GIP Receptor), also known as the glucose-dependent insulinotropic polypeptide receptor, is a protein which is present in different tissues; including in the pancreas, stomach, small intestine or adipose tissue, but also in several regions in the CNS. Adipocytes express GIP Receptors, where they modulate lipid accumulation.

Enteroendocrine K cells present in the duodenum and proximal jejunum secrete its natural ligand, the GIP, which is a 42–amino acid peptide. It has insulin-releasing and extrapancreatic glucoregulatory actions. GIP levels rise immediately after nutrient ingestion, leading to modest inhibitory effects on gastric acid secretion and gastrointestinal motility. cAMPNOMAD GIP Receptor Cell Line (GIPR Cell Line) allows the measurement of the activity of ligands, such as GIP.

Click HERE to know more about GIP Receptor.

  • Reference: P70529
  • Nomad Biosensors: cAMP
  • GeneBank Accesion Number: NP_000155
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice