cAMPNOMAD GLP-1 Receptor Cell Line

Reference: P70503

cAMPNOMAD GLP-1 Receptor Cell Line

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD GLP-1 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human GLP-1 Receptor (GLP1R) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD GLP-1 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to GLP-1, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

9.995,00

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The glucagon-like peptide-1 receptor (GLP1R) is a receptor protein which are present in beta cells of the pancreas; and also on neurons of the brain. It plays an important role in the control of blood sugar level by enhancing insulin secretion. It is a member of the glucagon receptor family of GPCRs. GLP1R comprises two domains, one extracellular (ECD) that binds the C-terminal helix of GLP-1, and one transmembrane (TMD) domain that binds the N-terminal region of GLP-1. In the TMD domain there is a fulcrum of polar residues that regulates the biased signaling of the receptor while the transmembrane helical boundaries and extracellular surface are a trigger for biased agonism. cAMPNOMAD GLP-1 Receptor Cell Line (GLP1R Cell Line) allows the measurement of the activity of ligands, such as GLP-1.

Click HERE to know more about GLP-1 Receptor.

  • Reference: P70503
  • Nomad Biosensors: cAMP
  • GeneBank Accesion Number: NM_002062.4
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice