cAMPNOMAD AVPR2 Cell Line

Reference: P70517

cAMPNOMAD AVPR2 Cell Line

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD AVPR2 Cell Line contains U2OS cells stably expressing the following constructs:

  • human AVPR2 with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

 

cAMPNOMAD AVPR2 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to [Arg8]‐AVP, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

9.995,00

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The activity of AVPR2 Receptor is mediated by G proteins which stimulate Adenylate cyclase – cAMP second messenger system. The AVPR2 receptor belongs to the same subfamily of G-protein coupled receptors of AVPR1A, AVPR1B and OTR receptors. AVPR2 is pressent in the kidney tubule, predominantly in the membrane of cells of the distal convoluted tubule and collecting ducts. AVPR2 is also present outside the kidney in vascular endothelium. Stimulation causes the release of von Willebrand factor and factor VIII from the endothelial cells. This is useful in the treatment of hemophilia A as well as Von Willebrand disease. cAMPNOMAD AVPR2 Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as [Arg8]‐AVP.

Click HERE to know more about AVPR2 Receptor.

  • Reference: P70517
  • Nomad Biosensors: cAMP
  • GeneBank Accesion Number: NM_000706.3
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice