cAMPNOMAD VPAC1 Receptor Cell Line

Reference: P70201

cAMPNOMAD VPAC1 Receptor Cell Line

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD VPAC1 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human VIP Receptor 1 (VPAC1R) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD VPAC1 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to PACAP-38, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

9.995,00

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Vasoactive intestinal polypeptide receptor 1, aka VPAC1, encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. VPAC1 is present in several tissues; such as brain, lung, prostate, peripheral blood leukocytes, liver, small intestine, heart, spleen, placenta, kidney, thymus and testis. VIP plays an imporatnt role in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. VIP acts in an autocrine fashion via VPAC1 to inhibit megakaryocyte proliferation and induce proplatelet formation. Patients with idiopathic achalasia show a significant difference in the distribution of SNPs affecting VIPR1. cAMPNOMAD VPAC1 Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as PACAP-38.

Click HERE to know more about VIPR1 Receptor.

  • Reference: P70201
  • Nomad Biosensors: cAMP
  • GeneBank Accesion Number: BC064424
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice