Epithelial Cell Medium – Serum Free (EpiCM-2), when used with Epithelial Cell Growth Supplement-2 (EpiCGS-2), is a complete medium designed for optimal growth of normal epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal epithelial cells in vitro.