Green Fluorescent B1 Bradykinin Receptor Cell Line

Reference: P30209

Green Fluorescent B1 Bradykinin Receptor Cell Line

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent B1 Bradykinin Receptor Cell Line contains CHO-K1 cells stably expressing BDKRB1 tagged with tGFP.  These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent B1 Bradykinin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate BDKRB1 activation and the following redistribution process inside the cells. When a ligand binds to the BDKRB1, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent BDKRB1 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring B1 Bradykinin Receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

6.950,00

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The bradykinin receptor family is a group of G-protein receptors whose principal ligand is the protein bradykinin. This gene encodes a receptor for bradykinin B1. Bradykinin, a 9 aa peptide, appears mainly in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Classical agonist of this receptor includes bradykinin1-8 (bradykinin with the first 8 amino acid) and antagonist includes [Leu8]-bradykinin1-8. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Green Fluorescent B1 Bradykinin Receptor from Innoprot allows the measurement of the activity and potency of ligands; such as bradykinin.

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  • Reference: P30209
  • DNA Accesion Number: NM_000710
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice