Green Fluorescent D1 Dopamine Receptor Cell Line

Reference: P30218

Green Fluorescent D1 Dopamine Receptor Cell Line

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent D1 Dopamine Receptor Cell Line contains SH-SY5Y cells stably expressing DRD1 Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent D1 Dopamine Receptor Cell Line allows to assay compounds, or analyze their capability to modulate DRD1 Receptor activation and the following redistribution process inside the cells. When a ligand binds to the DRD1 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent D1 Dopamine Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring DRD1 Receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

6.950,00

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This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. Specifically, it is is highest in the dorsal striatum (caudate and putamen) and ventral striatum (nucleus accumbens and olfatory tubercle). But lower levels of DRD1 mRNA expression occur in the basolateral amygdala, cerebral cortex, septum, thalamus, and hypothalamus. This G-protein-coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. Green Fluorescent D1 Dopamine Receptor Cell Line allows the measurement of the activity of ligands, such as Dopamine.

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  • Reference: P30218
  • DNA Accession Number: NM_000794
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice