Endothelial cells lining the microvasculature play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Intestinal microvascular endothelial cells also exhibit a strong immune response to LPS challenge. It also plays a critical regulatory role in gut inflammation. Pharmacological inhibition of NOS in activated intestinal microvascular endothelial cells resulted in a significant increase in leukocytes binding. Gene expression profile studies reveals that intestinal endothelial cells express biotinidase, which involves in biotin recycling. Human colonic microvascular endothelial cells allow performing systematic analyses of cytokine profiles with regard to mRNA expression and also protein secretion.
About turboGFP protein:
tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.