About Human Dermal Fibroblasts:
Cultures of human dermal fibroblasts are very useful for a wide range of cellular and molecular studies. This is mainly because fibroblasts are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in different organs are intrinsically different. Dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, human dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.
About turboGFP protein:
tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.