Green Fluorescent Immortalized Human Astrocytes

Reference: P10251-IM-tGFP

Green Fluorescent Immortalized Human Astrocytes

Green Fluorescent Immortalized Human Astrocytes (IM-HA-tGFP) provided by Innoprot have been developed by immortalizing primary human astrocytes with SV40 Large T antigen and stably transfected with tGFP.

Immortalized cells were controlled passaging side by side with the primary cells. These primary cells go into senescence after the 3rd passage while the SV40‐tranduced cells go beyond 30 passages.

Each vial of Green Fluorescent Immortalized Human Astrocytes contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HA-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HA-tGFP culture.


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About Human Astrocytes:

Astrocytes are the major cell type and among the most functionally diverse group of cells in the mammalian brain. They provide a variety of supportive functions to their partner neurons in the central nervous system (CNS); such as neuronal guidance during development, and nutritional and metabolic support throughout life. Astrocytes play important roles in various pathological processes. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also related to astrocyte surface molecule expression and trophic factor release. Much of what we have learned about astrocytes is from in vitro studies and immortalized astrocytes are useful tools for exploring the diverse properties of this cell type. Green Fluorescent Immortalized Human Astrocytes are also useful to stablish disease models for High Throughput and Hight Content Screening.


About turboGFP protein:

tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.

  • Reference: P10251-IM-tGFP
  • Size/Quantity: 1x106 cells / vial
  • Fluorescent Protein: turboGFP (excitation/emission max = 482/502 nm respectively)
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice

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