Green Fluorescent LS174T Cell Line are suitable for both, in vitro and in vivo experimentation.
About LS174T Cell line:
LS174T melanoma cell line comes from a Duke’s type B adenocarcinoma of the colon of a 58 year-old Caucasian female patient. LS 174T is a variant of LS 180 which has been in culture by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA). Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles. They are negative for p53 antigen expression, but positive for mRNA expression.
About turboGFP protein:
tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.