Green Fluorescent PAC1 Receptor Cell Line

Reference: P30214

Green Fluorescent PAC1 Receptor Cell Line

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent PAC1 Receptor Cell Line contains U2OS cells stably expressing PAC1 Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent PAC1 Receptor Cell Line allows to assay compounds, or analyze their capability to modulate PAC1 Receptor activation and the following redistribution process inside the cells. When a ligand binds to the PAC1 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent PAC1 Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring PAC1 Receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

6.950,00

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Pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1), is a protein that in humans is encoded by the ADCYAP1R1 gene. This receptor binds pituitary adenylate cyclase activating peptide. PAC1 is a membrane-associated protein and shares significant homology with members of the G-protein coupled class B glucagon/secretin receptor family. This receptor belongs to the family of  VIP and PACAP receptors. PAC1 receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and couples to adenylate cyclase. Alternative splicing of two exons of this gene generates four major splice variants. PAC1 is present in different tissue, such as adrenal medulla, pancreatic acini, uterus, myenteric plexus and brain. It is also present in the trigeminal, otic and superior cervical ganglia (prejunctional) and cerebral arteries (postjunctional). Green Fluorescent PAC1 Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as PACAP-38.

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  • Reference: P30214
  • DNA Accession Number: AY366498
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice